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ELK9723-96T, Cattle THP(Tamm–Horsfall Glycoprotein) ELISA Kit, 96T
2.963,10 RON
Cattle THP(Tamm–Horsfall Glycoprotein) ELISA Kit
SKU
ELK9723-96T
Alternative Names: THP; Uromucoid,Tamm-Horsfall Glycoprotein; Tamm-Horsfall urinary glycoprotein; Uromodulin; UMOD
Species: Cattle
Assay Type: Sandwich
Sensitivity: 0.263 ng/mL
Standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: serum, plasma, urine and other biological fluids
Assay length: 3.5h
Research Area: Kidney biomarker;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle THP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle THP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle THP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle THP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: THP; Uromucoid,Tamm-Horsfall Glycoprotein; Tamm-Horsfall urinary glycoprotein; Uromodulin; UMOD Species: Cattle Assay Type: Sandwich Sensitivity: 0.263 ng/mL Standard: 40 ng/mL Detection range: 0.63-40 ng/mL Sample type: serum, plasma, urine and other biological fluids Assay length: 3.5h Research Area: Kidney biomarker; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle THP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle THP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle THP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle THP in the samples is then determined by comparing the OD of the samples to the standard curve. |
