ELK9706-96T, Rat CREB(Cyclic AMP Response Element Binding Protein) ELISA Kit, 96T

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ELK9707-96T, Rat SESN3(Sestrin 3) ELISA Kit, 96T

ELK9707-96T, Rat SESN3(Sestrin 3) ELISA Kit, 96T

ELK9707-48T, Rat SESN3(Sestrin 3) ELISA Kit, 48T

2.142,00 RON

Rat SESN3(Sestrin 3) ELISA Kit

SKU
ELK9707-48T

Alternative Names: SEST3

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SESN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SESN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SESN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SESN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: SEST3

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SESN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SESN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SESN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SESN3 in the samples is then determined by comparing the OD of the samples to the standard curve.