Rabbit MC4R(Melanocortin receptor 4) ELISA Kit
Alternative Names: MC4R; Melanocortin receptor 4; MC4-R
Species: Rabbit
Assay Type: Sandwich
Sensitivity: 0.056 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: CD & Adhesion molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit MC4R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit MC4R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit MC4R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit MC4R in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 1.800,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: MC4R; Melanocortin receptor 4; MC4-R Species: Rabbit Assay Type: Sandwich Sensitivity: 0.056 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: Tissue homogenates and other biological fluids. Assay length: 3.5h Research Area: CD & Adhesion molecule; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit MC4R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit MC4R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit MC4R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit MC4R in the samples is then determined by comparing the OD of the samples to the standard curve. |