ELK9673-96T, Horse Presepsin(Presepsin) ELISA Kit, 96T

Alternative Names: soluble CD14 subtype; sCD14-ST; Presepsin

Species: Horse

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Sepsis

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse Presepsin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse Presepsin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse Presepsin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse Presepsin in the samples is then determined by comparing the OD of the samples to the standard curve.