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ELK9671-96T, Mouse CNCbl(Cyanocobalamin) ELISA Kit, 96T
2.963,10 RON
Mouse CNCbl(Cyanocobalamin) ELISA Kit
SKU
ELK9671-96T
Alternative Names: VB12; Vitamin B12; Cobalamin; α-5,6-Dimethylbenzimidazolyl Cobamidcyanide
Species: Mouse
Assay Type: Sandwich
Sensitivity: 49.7 pg/mL
Standard: 10000 pg/mL
Detection range: 156.25-10000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CNCbl. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CNCbl. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CNCbl, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CNCbl in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: VB12; Vitamin B12; Cobalamin; α-5,6-Dimethylbenzimidazolyl Cobamidcyanide Species: Mouse Assay Type: Sandwich Sensitivity: 49.7 pg/mL Standard: 10000 pg/mL Detection range: 156.25-10000 pg/mL Sample type: serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway;Nutrition metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CNCbl. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CNCbl. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CNCbl, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CNCbl in the samples is then determined by comparing the OD of the samples to the standard curve. |
