ELK9646-48T, F-E3(Free Estriol) ELISA Kit, 48T

ELK9646-48T, F-E3(Free Estriol) ELISA Kit, 48T

ELK9647-48T, Rabbit TPS(Tryptase) ELISA Kit, 48T

ELK9647-48T, Rabbit TPS(Tryptase) ELISA Kit, 48T

ELK9646-96T, F-E3(Free Estriol) ELISA Kit, 96T

2.475,20 RON

F-E3(Free Estriol) ELISA Kit

SKU
ELK9646-96T

Alternative Names: F-E3; Free Estriol

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.218 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Endocrinology;Reproductive science;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with F-E3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F-E3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-E3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: F-E3; Free Estriol

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.218 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Endocrinology;Reproductive science;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with F-E3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F-E3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-E3 in the samples is then determined by comparing the OD of the samples to the standard curve.