ELK9609-48T, Mouse D-LDH(D-LactateDehydrogenase) ELISA Kit, 48T

Alternative Names: DLD; LDHD; Probable D-lactate dehydrogenase;mitochondrial;

Species: Mouse

Assay Type: Sandwich

Sensitivity: 2.81 mIU/mL

Standard: 300 mIU/mL

Detection range: 4.69-300 mIU/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Signal Transduction;Cancer;Kits/ Lysates/ Other;Metabolism

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse D-LDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse D-LDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse D-LDH in the samples is then determined by comparing the OD of the samples to the standard curve.