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ELK9601-96T, Human HBsAg(HepatitisB surface antigen) ELISA Kit, 96T
2.963,10 RON
Human HBsAg(HepatitisB surface antigen) ELISA Kit
SKU
ELK9601-96T
Alternative Names: Australia antigen
Species: Human
Assay Type: Sandwich
Sensitivity: 0.19 ng/mL
Standard: 20 ng/mL
Detection range: 0.31-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Microbiology;Cancer
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HBsAg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBsAg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HBsAg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBsAg in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Australia antigen Species: Human Assay Type: Sandwich Sensitivity: 0.19 ng/mL Standard: 20 ng/mL Detection range: 0.31-20 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Microbiology;Cancer Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HBsAg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBsAg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HBsAg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBsAg in the samples is then determined by comparing the OD of the samples to the standard curve. |
