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ELK9590-96T, Human TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit, 96T
2.963,10 RON
Human TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit
SKU
ELK9590-96T
Alternative Names: ACP5;TRAP5b;SPENCDI
Species: Human
Assay Type: Sandwich
Sensitivity: 0.47 ng/mL
Standard: 50 ng/mL
Detection range: 0.78-50 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal Transduction;Signal Transduction;Metabolism
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: ACP5;TRAP5b;SPENCDI Species: Human Assay Type: Sandwich Sensitivity: 0.47 ng/mL Standard: 50 ng/mL Detection range: 0.78-50 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Signal Transduction;Signal Transduction;Metabolism Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve. |
