ELK9583-48T, TGF-b1(TransformingGrowth Factor Beta 1) ELISA Kit, 48T

Alternative Names: Cartilage-inducing factor;CED;Differentiation inhibiting factor;DPD1;LAP;Latency-associated peptide;Prepro transforming growth factor beta 1;TGF beta 1;TGF beta;TGF beta 1 protein;TGF-beta 1 protein;TGF-beta-1;TGF-beta-5;TGF-beta1;TGFB;Tgfb-1;tgfb1;TGFB1;TGFbeta;TGFbeta1;Transforming Growth Factor b1;Transforming Growth Factor beta 1;Transforming growth factor beta 1a;transforming growth factor beta-1;transforming growth factor;beta 1;Transforming Growth Factor-ß1

Species: General

Assay Type: Sandwich

Sensitivity: 0.10 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TGF-b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGF-b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TGF-b1 in the samples is then determined by comparing the OD of the samples to the standard curve.