ELK9576-48T, MEK(Methionine-Enkephalin) ELISA Kit, 48T

ELK9576-48T, MEK(Methionine-Enkephalin) ELISA Kit, 48T

ELK9577-48T, SP(Substance P) ELISA Kit, 48T

ELK9577-48T, SP(Substance P) ELISA Kit, 48T

ELK9576-96T, MEK(Methionine-Enkephalin) ELISA Kit, 96T

2.963,10 RON

MEK(Methionine-Enkephalin) ELISA Kit

SKU
ELK9576-96T

Alternative Names: Met-enkephalin; Opioid growth factor; OGF

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 75.00 pg/mL

Standard: 8000 pg/mL

Detection range: 125.00-8000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal Transduction

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with MEK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MEK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MEK in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Met-enkephalin; Opioid growth factor; OGF

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 75.00 pg/mL

Standard: 8000 pg/mL

Detection range: 125.00-8000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Signal Transduction

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with MEK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MEK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MEK in the samples is then determined by comparing the OD of the samples to the standard curve.