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ELK9564-96T, LTE4(Leukotriene E4) ELISA Kit, 96T
2.963,10 RON
LTE4(Leukotriene E4) ELISA Kit
SKU
ELK9564-96T
Alternative Names: Cysteinyl Leukotrienes E4
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 9.41 pg/mL
Standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Infection immunity;Autoimmunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LTE4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LTE4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LTE4 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Cysteinyl Leukotrienes E4 Species: General Assay Type: Competitive Inhibition Sensitivity: 9.41 pg/mL Standard: 2000 pg/mL Detection range: 31.25-2000 pg/mL Sample type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Metabolic pathway;Infection immunity;Autoimmunity; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LTE4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LTE4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LTE4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
