ELK9564-48T, LTE4(Leukotriene E4) ELISA Kit, 48T

ELK9564-48T, LTE4(Leukotriene E4) ELISA Kit, 48T

ELK9565-48T, Rat GFER(Growth Factor, Augmenter Of Liver Regeneration) ELISA Kit, 48T

ELK9565-48T, Rat GFER(Growth Factor, Augmenter Of Liver Regeneration) ELISA Kit, 48T

ELK9564-96T, LTE4(Leukotriene E4) ELISA Kit, 96T

2.963,10 RON

LTE4(Leukotriene E4) ELISA Kit

SKU
ELK9564-96T

Alternative Names: Cysteinyl Leukotrienes E4

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 9.41 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Infection immunity;Autoimmunity;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LTE4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LTE4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LTE4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Cysteinyl Leukotrienes E4

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 9.41 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Infection immunity;Autoimmunity;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LTE4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LTE4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LTE4 in the samples is then determined by comparing the OD of the samples to the standard curve.