ELK9550-96T, Human NCF2(Neutrophil Cytosolic Factor 2) ELISA Kit, 96T

ELK9550-96T, Human NCF2(Neutrophil Cytosolic Factor 2) ELISA Kit, 96T

ELK9551-96T, Guinea pig ADMA(Asymmetrical Dimethylarginine) ELISA Kit, 96T

ELK9551-96T, Guinea pig ADMA(Asymmetrical Dimethylarginine) ELISA Kit, 96T

ELK9551-48T, Guinea pig ADMA(Asymmetrical Dimethylarginine) ELISA Kit, 48T

1.814,75 RON

Guinea pig ADMA(Asymmetrical Dimethylarginine) ELISA Kit

SKU
ELK9551-48T

Alternative Names: N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine

Species: Guinea pig

Assay Type: Competitive Inhibition

Sensitivity: 4.51 ng/mL

Standard: 1000 ng/mL

Detection range: 15.63-1000 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Cardiovascular biology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Guinea pig ADMA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig ADMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig ADMA in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine

Species: Guinea pig

Assay Type: Competitive Inhibition

Sensitivity: 4.51 ng/mL

Standard: 1000 ng/mL

Detection range: 15.63-1000 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Cardiovascular biology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Guinea pig ADMA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig ADMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig ADMA in the samples is then determined by comparing the OD of the samples to the standard curve.