ELK9504-96T, Rat PYD(pyridinoline) ELISA Kit, 96T

ELK9504-96T, Rat PYD(pyridinoline) ELISA Kit, 96T

ELK9505-96T, Rat DPD(deoxypyridinoline) ELISA Kit, 96T

ELK9505-96T, Rat DPD(deoxypyridinoline) ELISA Kit, 96T

ELK9505-48T, Rat DPD(deoxypyridinoline) ELISA Kit, 48T

2.142,00 RON

Rat DPD(deoxypyridinoline) ELISA Kit

SKU
ELK9505-48T

Alternative Names: deoxypyridinoline

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.59 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Biochemical research

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DPD in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: deoxypyridinoline

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.59 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Biochemical research

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DPD in the samples is then determined by comparing the OD of the samples to the standard curve.