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ELK9498-48T, Human SMOC1(SPARC related modular calcium binding 1) ELISA Kit, 48T
2.142,00 RON
Human SMOC1(SPARC related modular calcium binding 1) ELISA Kit
SKU
ELK9498-48T
Alternative Names: Secreted modular calcium-binding protein 1
Species: Human
Assay Type: Sandwich
Sensitivity: 21.5 pg/mL
Standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: Serum, plasma, tissue homogenates
Assay length: 3.5h
Research Area: eye and limb development
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SMOC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SMOC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SMOC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SMOC1 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Secreted modular calcium-binding protein 1 Species: Human Assay Type: Sandwich Sensitivity: 21.5 pg/mL Standard: 5000 pg/mL Detection range: 78.13-5000 pg/mL Sample type: Serum, plasma, tissue homogenates Assay length: 3.5h Research Area: eye and limb development Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SMOC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SMOC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SMOC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SMOC1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
