Human WWOX(WW domain-containing oxidoreductase) ELISA Kit
Alternative Names: WWOX; FOR; WOX1; WW domain-containing oxidoreductase; Fragile site FRA16D oxidoreductase
Species: Human
Assay Type: Sandwich
Sensitivity: 0.115 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Cancer
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WWOX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WWOX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WWOX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WWOX in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 2.490,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: WWOX; FOR; WOX1; WW domain-containing oxidoreductase; Fragile site FRA16D oxidoreductase Species: Human Assay Type: Sandwich Sensitivity: 0.115 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 3.5h Research Area: Cancer Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WWOX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WWOX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WWOX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WWOX in the samples is then determined by comparing the OD of the samples to the standard curve. |