Limba
Romanian
Romanian
English|
ELK9489-96T, Cattle LDH(Lactate Dehydrogenase) ELISA Kit, 96T
2.963,10 RON
Cattle LDH(Lactate Dehydrogenase) ELISA Kit
SKU
ELK9489-96T
Alternative Names: Lactate Dehydrogenase
Species: Cattle
Assay Type: Sandwich
Sensitivity: 0.35 ng/mL
Standard: 50 ng/mL
Detection range: 0.79-50 ng/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle LDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle LDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle LDH in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Lactate Dehydrogenase Species: Cattle Assay Type: Sandwich Sensitivity: 0.35 ng/mL Standard: 50 ng/mL Detection range: 0.79-50 ng/mL Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle LDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle LDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle LDH in the samples is then determined by comparing the OD of the samples to the standard curve. |
