ELK9484-48T, SIP(Sphingosine 1 Phosphate) ELISA Kit, 48T

Alternative Names: EDG3; LPB3; S1P3; S1PR3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; Sphingosine 1-phosphate receptor Edg-3

Species: General

Assay Type: Sandwich

Sensitivity: 0.91 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SIP in the samples is then determined by comparing the OD of the samples to the standard curve.