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ELK9467-96T, Rat ADA(Adenosine Deaminase) ELISA Kit, 96T
2.963,10 RON
Rat ADA(Adenosine Deaminase) ELISA Kit
SKU
ELK9467-96T
Alternative Names: Adenosine Aminohydrolase
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.069 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ADA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ADA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADA in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Adenosine Aminohydrolase Species: Rat Assay Type: Sandwich Sensitivity: 0.069 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ADA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ADA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADA in the samples is then determined by comparing the OD of the samples to the standard curve. |
