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ELK9388-96T, Horse HSP90(Heat Shock Protein 90) ELISA Kit, 96T
2.963,10 RON
Horse HSP90(Heat Shock Protein 90) ELISA Kit
SKU
ELK9388-96T
Alternative Names: HtpG; Hsp-90 NTD; N-Terminal Domain
Species: Horse
Assay Type: Sandwich
Sensitivity: 1.19 ng/mL
Standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Infection immunity;Hematology;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse HSP90. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse HSP90. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse HSP90, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse HSP90 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: HtpG; Hsp-90 NTD; N-Terminal Domain Species: Horse Assay Type: Sandwich Sensitivity: 1.19 ng/mL Standard: 200 ng/mL Detection range: 3.13-200 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Signal transduction;Infection immunity;Hematology;Developmental science; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse HSP90. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse HSP90. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse HSP90, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse HSP90 in the samples is then determined by comparing the OD of the samples to the standard curve. |
