ELK9371-96T, Canine D2D(D-Dimer) ELISA Kit, 96T

Alternative Names: D 2 Dimer

Species: Canine

Assay Type: Sandwich

Sensitivity: 27.2 ng/mL

Standard: 5000 ng/mL

Detection range: 78.13-5000 ng/mL

Sample type: Plasma

Assay length: 3.5h

Research Area: Hematology;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Canine Canine D2D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Canine Canine D2D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Canine Canine D2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Canine Canine D2D in the samples is then determined by comparing the OD of the samples to the standard curve.