ELK9364-96T, Human sHLA-G(soluble human leucocyte antigen G) ELISA Kit, 96T

Alternative Names: sHLA-G

Species: Human

Assay Type: Sandwich

Sensitivity: 0.062 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Apoptosis;Tumor immunity;Infection immunity;Immune molecule;Hematology;Reproductive science;Immunodeficiency;Autoimmunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sHLA-G. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sHLA-G. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sHLA-G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sHLA-G in the samples is then determined by comparing the OD of the samples to the standard curve.