ELK9325-96T, Human RLN2(Relaxin 2) ELISA Kit, 96T

Alternative Names: RLN2; Prorelaxin H2; RLN2; RLXH2; bA12D24.1.1; bA12D24.1.1; bA12D24.1.2; bA12D24.1.2; H2; H2; relaxin 2; RLXH2; RLXH2

Species: Human

Assay Type: Sandwich

Sensitivity: 8.92 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;Reproductive science;Hormone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RLN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RLN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RLN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RLN2 in the samples is then determined by comparing the OD of the samples to the standard curve.