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ELK9321-96T, Human vHL(Von Hippel Lindau Tumor Suppressor) ELISA Kit, 96T
2.963,10 RON
Human vHL(Von Hippel Lindau Tumor Suppressor) ELISA Kit
SKU
ELK9321-96T
Alternative Names: HRCA1; RCA1; VHL1; Von Hippel-Lindau Syndrome
Species: Human
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human vHL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human vHL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human vHL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human vHL in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: HRCA1; RCA1; VHL1; Von Hippel-Lindau Syndrome Species: Human Assay Type: Sandwich Sensitivity: 0.113 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: Tissue homogenates and other biological fluids. Assay length: 3.5h Research Area: Tumor immunity; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human vHL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human vHL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human vHL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human vHL in the samples is then determined by comparing the OD of the samples to the standard curve. |
