ELK9314-48T, Human PGF2a(Prostaglandin F2 Alpha) ELISA Kit, 48T

Alternative Names: PGF2A; PGF2-A; PG-F2a

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 3.88 pg/mL

Standard: 800 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Cardiovascular biology;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PGF2a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve.