Limba
Romanian
Romanian
English|
ELK9257-48T, Pig ASMA(Anti-Smooth Muscle Antibody) ELISA Kit, 48T
1.814,75 RON
Pig ASMA(Anti-Smooth Muscle Antibody) ELISA Kit
SKU
ELK9257-48T
Alternative Names: ASM Ab; Anti Smooth Muscle Antibody
Species: Pig
Assay Type: Sandwich
Sensitivity: 2.9 ng/mL
Standard: 500 ng/mL
Detection range: 7.82-500 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig ASMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ASMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig ASMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ASMA in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.525,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: ASM Ab; Anti Smooth Muscle Antibody Species: Pig Assay Type: Sandwich Sensitivity: 2.9 ng/mL Standard: 500 ng/mL Detection range: 7.82-500 ng/mL Sample type: tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: Signal transduction Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig ASMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ASMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig ASMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ASMA in the samples is then determined by comparing the OD of the samples to the standard curve. |
