ELK9256-96T, Pig WNT1(Wingless Type MMTV Integration Site Family, Member 1) ELISA Kit, 96T

ELK9256-96T, Pig WNT1(Wingless Type MMTV Integration Site Family, Member 1) ELISA Kit, 96T

ELK9257-96T, Pig ASMA(Anti-Smooth Muscle Antibody) ELISA Kit, 96T

ELK9257-96T, Pig ASMA(Anti-Smooth Muscle Antibody) ELISA Kit, 96T

ELK9257-48T, Pig ASMA(Anti-Smooth Muscle Antibody) ELISA Kit, 48T

1.814,75 RON

Pig ASMA(Anti-Smooth Muscle Antibody) ELISA Kit

SKU
ELK9257-48T

Alternative Names: ASM Ab; Anti Smooth Muscle Antibody

Species: Pig

Assay Type: Sandwich

Sensitivity: 2.9 ng/mL

Standard: 500 ng/mL

Detection range: 7.82-500 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig ASMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ASMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig ASMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ASMA in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: ASM Ab; Anti Smooth Muscle Antibody

Species: Pig

Assay Type: Sandwich

Sensitivity: 2.9 ng/mL

Standard: 500 ng/mL

Detection range: 7.82-500 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig ASMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ASMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig ASMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ASMA in the samples is then determined by comparing the OD of the samples to the standard curve.