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ELK9249-96T, THB(Tetrahydrobiopterin) ELISA Kit, 96T
2.963,10 RON
THB(Tetrahydrobiopterin) ELISA Kit
SKU
ELK9249-96T
Alternative Names: Tetrahydrobiopterin; BH4; Kuvan; Sapropterin
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.51 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Genetic diseases
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with THB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to THB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of THB in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Tetrahydrobiopterin; BH4; Kuvan; Sapropterin Species: General Assay Type: Competitive Inhibition Sensitivity: 0.51 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Genetic diseases Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with THB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to THB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of THB in the samples is then determined by comparing the OD of the samples to the standard curve. |
