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ELK9228-48T, Dog ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit, 48T
2.142,00 RON
Dog ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit
SKU
ELK9228-48T
Alternative Names: Endocan
Species: Dog
Assay Type: Sandwich
Sensitivity: 6.2 pg/mL
Standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog ESM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog ESM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog ESM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog ESM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Endocan Species: Dog Assay Type: Sandwich Sensitivity: 6.2 pg/mL Standard: 1000 pg/mL Detection range: 15.63-1000 pg/mL Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Cardiovascular biology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog ESM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog ESM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog ESM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog ESM1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
