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ELK9185-96T, Pig DPD(deoxypyridinoline) ELISA Kit, 96T
2.963,10 RON
Pig DPD(deoxypyridinoline) ELISA Kit
SKU
ELK9185-96T
Alternative Names: deoxypyridinoline
Species: Pig
Assay Type: Sandwich
Sensitivity: 0.58 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Biochemical research
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig DPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig DPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig DPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig DPD in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: deoxypyridinoline Species: Pig Assay Type: Sandwich Sensitivity: 0.58 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Biochemical research Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig DPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig DPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig DPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig DPD in the samples is then determined by comparing the OD of the samples to the standard curve. |
