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ELK9154-96T, Mouse NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit, 96T
3.736,60 RON
Mouse NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit
SKU
ELK9154-96T
Alternative Names: NF kappa B p65delta3; NFKB3
Species: Mouse
Assay Type: Sandwich
Sensitivity: 12.6 pg/mL
Standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: serum, plasma, tissue homogenates
Assay length: 3.5h
Research Area: Immunology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: NF kappa B p65delta3; NFKB3 Species: Mouse Assay Type: Sandwich Sensitivity: 12.6 pg/mL Standard: 2000 pg/mL Detection range: 31.25-2000 pg/mL Sample type: serum, plasma, tissue homogenates Assay length: 3.5h Research Area: Immunology Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve. |
