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ELK9142-96T, Human dsDNA(anti-double stranded DNA antibody IgG) ELISA Kit, 96T
2.963,10 RON
Human dsDNA(anti-double stranded DNA antibody IgG) ELISA Kit
SKU
ELK9142-96T
Alternative Names: dsDNA Ab IgG
Species: Human
Assay Type: Sandwich
Sensitivity: 0.096 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, urine and other biological fluids
Assay length: 3.5h
Research Area: disease
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human dsDNA IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human dsDNA IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human dsDNA IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human dsDNA IgG in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: dsDNA Ab IgG Species: Human Assay Type: Sandwich Sensitivity: 0.096 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: serum, plasma, urine and other biological fluids Assay length: 3.5h Research Area: disease Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human dsDNA IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human dsDNA IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human dsDNA IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human dsDNA IgG in the samples is then determined by comparing the OD of the samples to the standard curve. |
