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ELK9078-96T, Rat PC(Protein Carbonyl) ELISA Kit, 96T
2.963,10 RON
Rat PC(Protein Carbonyl) ELISA Kit
SKU
ELK9078-96T
Alternative Names: Protein Carbonyl
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.114 nmol/mL
Standard: 20 nmol/mL
Detection range: 0.32-20 nmol/mL
Sample type: Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Lysate
Assay length: 3.5h
Research Area: Metabolic pathway;Hepatology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PC in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Protein Carbonyl Species: Rat Assay Type: Sandwich Sensitivity: 0.114 nmol/mL Standard: 20 nmol/mL Detection range: 0.32-20 nmol/mL Sample type: Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Lysate Assay length: 3.5h Research Area: Metabolic pathway;Hepatology;Hormone metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PC in the samples is then determined by comparing the OD of the samples to the standard curve. |
