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ELK9070-96T, Rat HMGCS(Hydroxymethylglutaryl Coenzyme A Synthase) ELISA Kit, 96T
2.963,10 RON
Rat HMGCS(Hydroxymethylglutaryl Coenzyme A Synthase) ELISA Kit
SKU
ELK9070-96T
Alternative Names: HMGCS1; 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase 1,Soluble; HMG-CoA Synthase
Species: Rat
Assay Type: Sandwich
Sensitivity: 5.7 pg/mL
Standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HMGCS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HMGCS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HMGCS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HMGCS in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: HMGCS1; 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase 1,Soluble; HMG-CoA Synthase Species: Rat Assay Type: Sandwich Sensitivity: 5.7 pg/mL Standard: 1000 pg/mL Detection range: 15.63-1000 pg/mL Sample type: Tissue homogenates and other biological fluids. Assay length: 3.5h Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HMGCS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HMGCS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HMGCS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HMGCS in the samples is then determined by comparing the OD of the samples to the standard curve. |
