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ELK9062-96T, Rat CHRNA7(Neuronal Acetylcholine Receptor Subunit Alpha 7) ELISA Kit, 96T
2.475,20 RON
Rat CHRNA7(Neuronal Acetylcholine Receptor Subunit Alpha 7) ELISA Kit
SKU
ELK9062-96T
Alternative Names: NACHRA7; CHRNA7-2
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.056 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Neuro science
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CHRNA7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CHRNA7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CHRNA7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CHRNA7 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: NACHRA7; CHRNA7-2 Species: Rat Assay Type: Sandwich Sensitivity: 0.056 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Neuro science Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CHRNA7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CHRNA7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CHRNA7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CHRNA7 in the samples is then determined by comparing the OD of the samples to the standard curve. |
