ELK9057-96T, Rat MHCII(Major histocompatibility complex class II) ELISA Kit, 96T

ELK9057-96T, Rat MHCII(Major histocompatibility complex class II) ELISA Kit, 96T

ELK9058-96T, Human TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit, 96T

ELK9058-96T, Human TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit, 96T

ELK9058-48T, Human TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit, 48T

2.142,00 RON

Human TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit

SKU
ELK9058-48T

Alternative Names: TPO Ab; TPO-Ab

Species: Human

Assay Type: Sandwich

Sensitivity: 1.19 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPO-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: TPO Ab; TPO-Ab

Species: Human

Assay Type: Sandwich

Sensitivity: 1.19 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPO-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.