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ELK9040-96T, Human Endocab IgG ELISA Kit, 96T
2.963,10 RON
Human Endocab IgG ELISA Kit
SKU
ELK9040-96T
Alternative Names: Endocab IgG; Endocab
Species: Human
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: CD & Adhesion molecule
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Endocab IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Endocab IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Endocab IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Endocab IgG in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Endocab IgG; Endocab Species: Human Assay Type: Sandwich Sensitivity: 0.059 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: CD & Adhesion molecule Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Endocab IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Endocab IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Endocab IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Endocab IgG in the samples is then determined by comparing the OD of the samples to the standard curve. |
