ELK9034-48T, Human PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit, 48T

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ELK9034-96T, Human PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit, 96T

2.963,10 RON

Human PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit

SKU
ELK9034-96T

Alternative Names: PHOSPHATIDYLINOSITOL GLYCAN; Phosphatidylinositol glycan biosynthesis class F protein; Phosphatidylinositol-glycan biosynthesis class F protein; PIG F; PIG-F; PIGF; PIGF_HUMAN; PIGF1

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 6.2 ng/mL

Standard: 1000 ng/mL

Detection range: 15.6-1000 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: PHOSPHATIDYLINOSITOL GLYCAN; Phosphatidylinositol glycan biosynthesis class F protein; Phosphatidylinositol-glycan biosynthesis class F protein; PIG F; PIG-F; PIGF; PIGF_HUMAN; PIGF1

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 6.2 ng/mL

Standard: 1000 ng/mL

Detection range: 15.6-1000 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 2h

Research Area: Signal transduction;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F in the samples is then determined by comparing the OD of the samples to the standard curve.