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ELK8999-96T, Cattle SPATA4 (Spermatogenesis-associated protein 4) ELISA Kit, 96T
2.963,10 RON
Cattle SPATA4 (Spermatogenesis-associated protein 4) ELISA Kit
SKU
ELK8999-96T
Alternative Names: SPATA4
Species: Cattle
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: serum, plasma, tissue homogenates
Assay length: 3.5h
Research Area: Reproductive science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle SPATA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle SPATA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle SPATA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle SPATA4 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: SPATA4 Species: Cattle Assay Type: Sandwich Sensitivity: 0.061 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: serum, plasma, tissue homogenates Assay length: 3.5h Research Area: Reproductive science; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle SPATA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle SPATA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle SPATA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle SPATA4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
