ELK8997-48T, Rat PIIANP(Type IIA Collagen N-Propeptide) ELISA Kit, 48T

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ELK8997-96T, Rat PIIANP(Type IIA Collagen N-Propeptide) ELISA Kit, 96T

2.963,10 RON

Rat PIIANP(Type IIA Collagen N-Propeptide) ELISA Kit

SKU
ELK8997-96T

Alternative Names: PIIANP

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 6.2 ng/mL

Standard: 1000 ng/mL

Detection range: 15.6-1000 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Hepatology;Bone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PIIANP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIIANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIIANP in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: PIIANP

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 6.2 ng/mL

Standard: 1000 ng/mL

Detection range: 15.6-1000 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Hepatology;Bone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PIIANP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIIANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIIANP in the samples is then determined by comparing the OD of the samples to the standard curve.