ELK8977-96T, Simian SAA(Serum Amyloid A) ELISA Kit, 96T

Alternative Names: SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

Species: Simian

Assay Type: Sandwich

Sensitivity: 0.37 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Infection immunity;Kidney biomarker;Hepatology;Urology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian SAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian SAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian SAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian SAA in the samples is then determined by comparing the OD of the samples to the standard curve.