ELK8936-48T, Human CYP2A6(Cytochrome P450 2A6) ELISA Kit, 48T

Alternative Names: CYP2A6; Cytochrome P450; I; Cytochrome P450; I; Cytochrome P450 IIA3; CYPIIA6; Coumarin 7-hydroxylase; CYP2A3

Species: Human

Assay Type: Sandwich

Sensitivity: 24.9 pg/mL

Standard: 4000 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: CD & Adhesion molecule;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYP2A6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYP2A6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYP2A6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYP2A6 in the samples is then determined by comparing the OD of the samples to the standard curve.