ELK8920-48T, Human hs Ab1-42(high sensitivity Amyloid Beta Peptide 1-42) ELISA Kit, 48T

Alternative Names: Amyloid Beta Peptide 1-42;

Species: Human

Assay Type: Sandwich

Sensitivity: 1.35 pg/mL

Standard: 300 pg/mL

Detection range: 4.69-300 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.