ELK8912-48T, Rat SCARD1(Scavenger Receptor Class D Member 1) ELISA Kit, 48T

Alternative Names: CD68; GP110; Macrosialin; Macrophage Antigen CD68

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.119 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SCARD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCARD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SCARD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SCARD1 in the samples is then determined by comparing the OD of the samples to the standard curve.