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ELK8905-48T, Rat TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit, 48T
2.142,00 RON
Rat TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit
SKU
ELK8905-48T
Alternative Names: TBARS; Thiobarbituric Acid Reactive Substance
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 3.55 nmol/mL
Standard: 400 nmol/mL
Detection range: 6.25-400 nmol/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length: 2h
Research Area: Metabolic pathway;Hepatology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TBARS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TBARS in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: TBARS; Thiobarbituric Acid Reactive Substance Species: Rat Assay Type: Competitive Inhibition Sensitivity: 3.55 nmol/mL Standard: 400 nmol/mL Detection range: 6.25-400 nmol/mL Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay length: 2h Research Area: Metabolic pathway;Hepatology;Hormone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TBARS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TBARS in the samples is then determined by comparing the OD of the samples to the standard curve. |
