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ELK8897-48T, Rat Total cholesterol (TC) ELISA Kit, 48T
1.814,75 RON
Rat Total cholesterol (TC) ELISA Kit
SKU
ELK8897-48T
Alternative Names: TC
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 2.63 µg/mL
Standard: 500 µg/mL
Detection range: 7.82-500 µg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TC in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.525,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: TC Species: Rat Assay Type: Competitive Inhibition Sensitivity: 2.63 µg/mL Standard: 500 µg/mL Detection range: 7.82-500 µg/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Metabolic pathway;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TC in the samples is then determined by comparing the OD of the samples to the standard curve. |
