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ELK8894-96T, Zebrafish Cor(Cortisol) ELISA Kit, 96T
2.475,20 RON
Zebrafish Cor(Cortisol) ELISA Kit
SKU
ELK8894-96T
Alternative Names: Hydrocortisone
Species: Zebrafish
Assay Type: Competitive Inhibition
Sensitivity: 0.93 ng/mL
Standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: serum, plasma, urine and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish Cor protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish Cor in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.080,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Hydrocortisone Species: Zebrafish Assay Type: Competitive Inhibition Sensitivity: 0.93 ng/mL Standard: 200 ng/mL Detection range: 3.13-200 ng/mL Sample type: serum, plasma, urine and other biological fluids Assay length: 2h Research Area: Metabolic pathway;Endocrinology;Hormone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish Cor protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish Cor in the samples is then determined by comparing the OD of the samples to the standard curve. |
