ELK8892-96T, Mouse C1s(Complement component 1, s subcomponent) ELISA Kit, 96T

Alternative Names: Basic proline rich peptide IB 1 ; C1 esterase ; C1S ;  Complement C1s subcomponent ; Complement C1s subcomponent heavy chain ; Complement C1s subcomponent light chain ; Complement component 1 s subcomponent ; Complement component 1 subcomponent s ; FLJ44757

Species: Mouse

Assay Type: Sandwich

Sensitivity: 6.65 ng/mL

Standard: 1000 ng/mL

Detection range: 15.63-1000 ng/mL

Sample type: serum, plasma, tissue homogenates, and other biological fluids

Assay length: 3.5h

Research Area: Immunology

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C1s. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C1s. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C1s, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C1s in the samples is then determined by comparing the OD of the samples to the standard curve.