ELK8890-96T, Mouse Lptn/LTN/XCL1 (Lymphotactin) ELISA Kit, 96T

Alternative Names: Xcl1 ; Lptn ; Ltn ; Scyc1 ; Lymphotactin ; C motif chemokine 1 ; Cytokine SCM-1 ; Lymphotaxin ; Small-inducible cytokine C1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 3.27 pg/mL

Standard: 500 pg/mL

Detection range: 7.82-500 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;Dermatology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Lptn/LTN/XCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Lptn/LTN/XCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Lptn/LTN/XCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Lptn/LTN/XCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.