ELK8882-48T, Cattle MBP(Myelin Basic Protein) ELISA Kit, 48T

Alternative Names: Myelin A1 protein; Myelin membrane encephalitogenic protein

Species: Cattle

Assay Type: Sandwich

Sensitivity: 0.147 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Infection immunity;Neuro science;Autoimmunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle MBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle MBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle MBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle MBP in the samples is then determined by comparing the OD of the samples to the standard curve.