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ELK8878-96T, High Sensitivity Human I-PTH(IntactParathormone) ELISA Kit, 96T
3.736,60 RON
High Sensitivity Human I-PTH(IntactParathormone) ELISA Kit
SKU
ELK8878-96T
Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone
Species: Human
Assay Type: Sandwich
Sensitivity: 0.9 pg/mL
Standard: 200 pg/mL
Detection range: 3.13-200 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone Species: Human Assay Type: Sandwich Sensitivity: 0.9 pg/mL Standard: 200 pg/mL Detection range: 3.13-200 pg/mL Sample type: serum, plasma and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway;Endocrinology;Hormone metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve. |
